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reg3γ antibody (WuXi AppTec)

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WuXi AppTec reg3γ antibody
New <t>Reg3γ</t> antibody lot workup for mice and rats.

Reg3γ Antibody, supplied by WuXi AppTec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/reg3%CE%B3+antibody/reg3%CE%B3+antibody/pmc06749974-79-1-6
Average 90 stars, based on 1 article reviews

reg3γ antibody - by Bioz Stars, 2026-10

90/100 stars

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1) Product Images from "Detection of Reg3γ by Immunohistochemistry in Cerulein-Induced Model of Acute Pancreatic Injury in Mice and Rats"

Article Title: Detection of Reg3γ by Immunohistochemistry in Cerulein-Induced Model of Acute Pancreatic Injury in Mice and Rats

Journal: Pancreas

doi: 10.1097/MPA.0000000000001382

Figure Legend Snippet: New Reg3γ antibody lot workup for mice and rats.

Techniques Used:

Rat control comparison: rat positive control slides from all experimental runs. All control slides are nonserial sections from the same tissue block. A1–A6, control slides for 6- and 24-hour animals (first antibody lot). B1–B4, control slides for 48-hour animals (second antibody lot). Tissue outlined in yellow was used for analysis across lots and runs to yield percent positivity of Reg3γ and average intensity of positive pixels using Aperio Positive Pixel Count Algorithm v9. Percent positivity (P) of Reg3γ and average intensity (I) are listed on each image. Magnification 1.25×. Scale bar, 1000 μm.

Figure Legend Snippet: Rat control comparison: rat positive control slides from all experimental runs. All control slides are nonserial sections from the same tissue block. A1–A6, control slides for 6- and 24-hour animals (first antibody lot). B1–B4, control slides for 48-hour animals (second antibody lot). Tissue outlined in yellow was used for analysis across lots and runs to yield percent positivity of Reg3γ and average intensity of positive pixels using Aperio Positive Pixel Count Algorithm v9. Percent positivity (P) of Reg3γ and average intensity (I) are listed on each image. Magnification 1.25×. Scale bar, 1000 μm.

Techniques Used: Positive Control, Blocking Assay

Mouse and rat pancreas, Reg3γ IHC by treatment group 6 hours postdose. Mouse: (A) control, (B) low, (C) medium, and (D) high. Rat: (E) control, (F) low, (G) medium, and (H) high. Reg3γ-labeled staining, 3,3'-diaminobenzidine (brown); counterstain, hematoxylin (blue). Magnification 40×. Scale bar, 50 μm.

Figure Legend Snippet: Mouse and rat pancreas, Reg3γ IHC by treatment group 6 hours postdose. Mouse: (A) control, (B) low, (C) medium, and (D) high. Rat: (E) control, (F) low, (G) medium, and (H) high. Reg3γ-labeled staining, 3,3'-diaminobenzidine (brown); counterstain, hematoxylin (blue). Magnification 40×. Scale bar, 50 μm.

Techniques Used: Labeling, Staining

Mouse and rat pancreas, Reg3γ IHC by treatment group 48 hours postdose. Mouse: (A) control, (B) low, (C) medium, and (D) high. Rat: (E) control, (F) low, (G) medium, and (H) high. Reg3γ-labeled staining, 3,3'-diaminobenzidine (brown); counterstain, hematoxylin (blue). Magnification 40×. Scale bar, 50 μm.

Figure Legend Snippet: Mouse and rat pancreas, Reg3γ IHC by treatment group 48 hours postdose. Mouse: (A) control, (B) low, (C) medium, and (D) high. Rat: (E) control, (F) low, (G) medium, and (H) high. Reg3γ-labeled staining, 3,3'-diaminobenzidine (brown); counterstain, hematoxylin (blue). Magnification 40×. Scale bar, 50 μm.

Techniques Used: Labeling, Staining

Figure Legend Snippet: Mouse Reg3γ IHC Image Analysis: Percent Positivity

Techniques Used:

Figure Legend Snippet: Rat Reg3γ IHC Image Analysis: Percent Positivity

Techniques Used:

Figure Legend Snippet: Mouse and Rat Reg3γ Percent Positive IHC Correlations to Histology Scores

Techniques Used:

Figure Legend Snippet: Rat Reg3γ Gene Expression Correlations to Reg3γ % Positivity and Histology Scores

Techniques Used: Expressing

Figure Legend Snippet: Rat Reg3γ Differential Gene Expression Statistics

Techniques Used: Expressing

Incubation:

Article Title: Detection of Reg3γ by Immunohistochemistry in Cerulein-Induced Model of Acute Pancreatic Injury in Mice and Rats
Article Snippet: Before antibody application, nonspecific staining was blocked using Antibody Block (Ventana Medical). .. The rabbit polyclonal Reg3γ antibody (AP5606c; Abgent, San Diego, Calif) was then incubated on the tissue sections for 1 hour at 3 μg/mL, or 10 μg/mL for 48-hour mice and 8.3 μg/mL for 48-hour rats. .. The primary antibody was then labeled with a biotinylated goat antirabbit antibody (Vector Laboratories, Burlingame, Calif).

Control:

Article Title: Innate Stat3-mediated induction of the antimicrobial protein Reg3γ is required for host defense against MRSA pneumonia
Article Snippet: For binding, overnight cultures of SA USA300 were resuspended in PBS containing recombinant mouse Reg3γ-Fc (provided by W. Ouyang, Genentech, South San Francisco, CA; ) or IgG2a isotype control (BD) for 1 h, followed by goat anti–mouse IgG2a–Alexa Fluor 488 (Invitrogen) for 30 min. Bacteria were fixed with 2% formaldehyde, and flow cytometry was conducted on a FACSCalibur (BD). .. To assess growth kinetics, overnight cultures were diluted 1:500 in TSB containing isotype control, Reg3γ-Fc, or Reg3γ-Fc with Reg3γ antibody (Abgent), and OD600 readings were measured at hourly intervals. ..

Image Search Results

Journal: Immunologic Research

Article Title: Baicalin restores innate lymphoid immune imbalance during exacerbation of COPD

doi: 10.1007/s12026-025-09629-2

Figure Lengend Snippet: Primer sequences

Article Snippet: Reg3γ antibody (A2146) and Recombinant mouse IL- 22 (RP02942) were purchased from ABclonal Technology Co.,Ltd. (Wuhan, Hubei, China).

Techniques:

CS and LPS-induced ECOPD mice show an upregulation of IL- 22/Reg3γ axis. a Expression of IL- 22 in both protein and mRNA levels in BALF and lung tissues. b Expression of IL- 22, GATA3, and Reg3γ in lung tissues, assessed using immuohistochemical staining. c Expression of IL- 22, GATA3, and Reg3γ in lung tissues, assessed using western blotting. Data are mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001. LTH: lung tissue homogenate

Journal: Immunologic Research

Article Title: Baicalin restores innate lymphoid immune imbalance during exacerbation of COPD

doi: 10.1007/s12026-025-09629-2

Figure Lengend Snippet: CS and LPS-induced ECOPD mice show an upregulation of IL- 22/Reg3γ axis. a Expression of IL- 22 in both protein and mRNA levels in BALF and lung tissues. b Expression of IL- 22, GATA3, and Reg3γ in lung tissues, assessed using immuohistochemical staining. c Expression of IL- 22, GATA3, and Reg3γ in lung tissues, assessed using western blotting. Data are mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001. LTH: lung tissue homogenate

Article Snippet: Reg3γ antibody (A2146) and Recombinant mouse IL- 22 (RP02942) were purchased from ABclonal Technology Co.,Ltd. (Wuhan, Hubei, China).

Techniques: Expressing, Staining, Western Blot

Baicalin inhibits the IL- 22/Reg3γ axis in ECOPD mice. a The effect of baicalin on the expression of IL- 22 in lung tissue in ECOPD mice, assessed using ELISA and qPCR. b The effect of baicalin on the expression of IL- 22, GATA3, and Reg3γ in lung tissue in ECOPD mice, assessed using immuohistochemical staining. c The effect of baicalin on the expression of IL- 22 and Reg3γ in lung tissue in ECOPD mice, assessed using western blotting. d The effect of baicalin on the number of IL- 22-producing ILC3 s in lung tissue in ECOPD mice. e The effect of baicalin on the production of IL- 22 in isolated human blood ILC3 s. Data are mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. BA: baicalin; LTH: lung tissue homogenate

Journal: Immunologic Research

Article Title: Baicalin restores innate lymphoid immune imbalance during exacerbation of COPD

doi: 10.1007/s12026-025-09629-2

Figure Lengend Snippet: Baicalin inhibits the IL- 22/Reg3γ axis in ECOPD mice. a The effect of baicalin on the expression of IL- 22 in lung tissue in ECOPD mice, assessed using ELISA and qPCR. b The effect of baicalin on the expression of IL- 22, GATA3, and Reg3γ in lung tissue in ECOPD mice, assessed using immuohistochemical staining. c The effect of baicalin on the expression of IL- 22 and Reg3γ in lung tissue in ECOPD mice, assessed using western blotting. d The effect of baicalin on the number of IL- 22-producing ILC3 s in lung tissue in ECOPD mice. e The effect of baicalin on the production of IL- 22 in isolated human blood ILC3 s. Data are mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. BA: baicalin; LTH: lung tissue homogenate

Article Snippet: Reg3γ antibody (A2146) and Recombinant mouse IL- 22 (RP02942) were purchased from ABclonal Technology Co.,Ltd. (Wuhan, Hubei, China).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Staining, Western Blot, Isolation

Figure 1. IEC-derived GPR65 preserves intestinal antimicrobial activity. (a-c) qPCR analysis of the indicated genes in IECs from the large bowels of Gpr65fl/fl and Gpr65ΔIEC mice. (d-f) qPCR analysis of the indicated genes in IECs from the small bowels of Gpr65fl/fl and Gpr65ΔIEC mice. (g) representative immunofluorescence staining of REG3γ in both large and small bowels from Gpr65fl/fl and Gpr65ΔIEC

Journal: Gut microbes

Article Title: Intestinal epithelial pH-sensing receptor GPR65 maintains mucosal homeostasis via regulating antimicrobial defense and restrains gut inflammation in inflammatory bowel disease.

doi: 10.1080/19490976.2023.2257269

Figure Lengend Snippet: Figure 1. IEC-derived GPR65 preserves intestinal antimicrobial activity. (a-c) qPCR analysis of the indicated genes in IECs from the large bowels of Gpr65fl/fl and Gpr65ΔIEC mice. (d-f) qPCR analysis of the indicated genes in IECs from the small bowels of Gpr65fl/fl and Gpr65ΔIEC mice. (g) representative immunofluorescence staining of REG3γ in both large and small bowels from Gpr65fl/fl and Gpr65ΔIEC

Article Snippet: Primary antibodies against REG3γ (Cat: ab198216), β-actin (Cat: sc-8432), and GPR65 (Cat: PA5–111835) were purchased from Abcam (Cambridge, UK), Santa Cruz Biotechnology (Dallas, TX, USA), and Thermo Fisher Scientific, respectively.

Techniques: Derivative Assay, Activity Assay, Immunofluorescence, Staining

AP patients and experimental AP mice presented Paneth cell defects. (A) Histopathological changes and mean numbers of Paneth cells per crypt of duodenal mucosa specimens were assessed by H&E staining. Original magnification, ×200 ( n = 7 to 14 individuals per group). (B) Lysozyme expression (green) was assessed in Paneth cells of duodenal mucosa specimens by immunofluorescence (magnification, ×200) and lysozyme-positive/DAPI + quantification. (C to K) The mRNA expression levels of lysozyme (C), HD6 (D), HD5 (E), Reg3γ (F), Ang4 (G), sPLA2 (H), TGFβ (I), Wnt3a (J), and Lgr5 (K) were assessed. (L) Lysozyme expression (green) (magnification, ×200) and lysozyme-positive/DAPI + quantification in three AP models. (M) Mean number of Paneth cells per crypt in AP models. The data are presented as the means ± SD. ns, no significant difference; *, P ≤ 0.05.

Journal: mSystems

Article Title: Paneth Cells Protect against Acute Pancreatitis via Modulating Gut Microbiota Dysbiosis

doi: 10.1128/msystems.01507-21

Figure Lengend Snippet: AP patients and experimental AP mice presented Paneth cell defects. (A) Histopathological changes and mean numbers of Paneth cells per crypt of duodenal mucosa specimens were assessed by H&E staining. Original magnification, ×200 ( n = 7 to 14 individuals per group). (B) Lysozyme expression (green) was assessed in Paneth cells of duodenal mucosa specimens by immunofluorescence (magnification, ×200) and lysozyme-positive/DAPI + quantification. (C to K) The mRNA expression levels of lysozyme (C), HD6 (D), HD5 (E), Reg3γ (F), Ang4 (G), sPLA2 (H), TGFβ (I), Wnt3a (J), and Lgr5 (K) were assessed. (L) Lysozyme expression (green) (magnification, ×200) and lysozyme-positive/DAPI + quantification in three AP models. (M) Mean number of Paneth cells per crypt in AP models. The data are presented as the means ± SD. ns, no significant difference; *, P ≤ 0.05.

Article Snippet: The membrane was blocked with 3% bovine serum albumin (BSA) for 1 h and incubated with primary antibodies, diluted in primary antibody dilution buffer (Epizyme Biotech, China), against Lgr5 (catalog number A10545; Abclonal, China), lysozyme (catalog number A0099; Dako, Denmark), Reg3γ (catalog number sc-377038; Santa Cruz Biotechnology, USA), Defa5 (catalog number A18208; Abclonal, China), Ang4 (catalog number sc-377497; Santa Cruz Biotechnology, USA), and sPLA2 (catalog number sc-58363; Santa Cruz Biotechnology, USA) overnight at 4°C.

Techniques: Staining, Expressing, Immunofluorescence

Journal: Pancreas

Article Title: Detection of Reg3γ by Immunohistochemistry in Cerulein-Induced Model of Acute Pancreatic Injury in Mice and Rats

doi: 10.1097/MPA.0000000000001382

Figure Lengend Snippet: New Reg3γ antibody lot workup for mice and rats.

Article Snippet: The rabbit polyclonal Reg3γ antibody (AP5606c; Abgent, San Diego, Calif) was then incubated on the tissue sections for 1 hour at 3 μg/mL, or 10 μg/mL for 48-hour mice and 8.3 μg/mL for 48-hour rats.

Techniques:

Journal: Pancreas

Article Title: Detection of Reg3γ by Immunohistochemistry in Cerulein-Induced Model of Acute Pancreatic Injury in Mice and Rats

doi: 10.1097/MPA.0000000000001382

Figure Lengend Snippet: Rat control comparison: rat positive control slides from all experimental runs. All control slides are nonserial sections from the same tissue block. A1–A6, control slides for 6- and 24-hour animals (first antibody lot). B1–B4, control slides for 48-hour animals (second antibody lot). Tissue outlined in yellow was used for analysis across lots and runs to yield percent positivity of Reg3γ and average intensity of positive pixels using Aperio Positive Pixel Count Algorithm v9. Percent positivity (P) of Reg3γ and average intensity (I) are listed on each image. Magnification 1.25×. Scale bar, 1000 μm.

Techniques: Positive Control, Blocking Assay

Journal: Pancreas

Article Title: Detection of Reg3γ by Immunohistochemistry in Cerulein-Induced Model of Acute Pancreatic Injury in Mice and Rats

doi: 10.1097/MPA.0000000000001382

Figure Lengend Snippet: Mouse and rat pancreas, Reg3γ IHC by treatment group 6 hours postdose. Mouse: (A) control, (B) low, (C) medium, and (D) high. Rat: (E) control, (F) low, (G) medium, and (H) high. Reg3γ-labeled staining, 3,3'-diaminobenzidine (brown); counterstain, hematoxylin (blue). Magnification 40×. Scale bar, 50 μm.

Techniques: Labeling, Staining

Journal: Pancreas

Article Title: Detection of Reg3γ by Immunohistochemistry in Cerulein-Induced Model of Acute Pancreatic Injury in Mice and Rats

doi: 10.1097/MPA.0000000000001382

Figure Lengend Snippet: Mouse and rat pancreas, Reg3γ IHC by treatment group 48 hours postdose. Mouse: (A) control, (B) low, (C) medium, and (D) high. Rat: (E) control, (F) low, (G) medium, and (H) high. Reg3γ-labeled staining, 3,3'-diaminobenzidine (brown); counterstain, hematoxylin (blue). Magnification 40×. Scale bar, 50 μm.

Techniques: Labeling, Staining

Journal: Pancreas

Article Title: Detection of Reg3γ by Immunohistochemistry in Cerulein-Induced Model of Acute Pancreatic Injury in Mice and Rats

doi: 10.1097/MPA.0000000000001382

Figure Lengend Snippet: Mouse Reg3γ IHC Image Analysis: Percent Positivity

Techniques:

Journal: Pancreas

Article Title: Detection of Reg3γ by Immunohistochemistry in Cerulein-Induced Model of Acute Pancreatic Injury in Mice and Rats

doi: 10.1097/MPA.0000000000001382

Figure Lengend Snippet: Rat Reg3γ IHC Image Analysis: Percent Positivity

Techniques:

Journal: Pancreas

Article Title: Detection of Reg3γ by Immunohistochemistry in Cerulein-Induced Model of Acute Pancreatic Injury in Mice and Rats

doi: 10.1097/MPA.0000000000001382

Figure Lengend Snippet: Mouse and Rat Reg3γ Percent Positive IHC Correlations to Histology Scores

Techniques:

Journal: Pancreas

Article Title: Detection of Reg3γ by Immunohistochemistry in Cerulein-Induced Model of Acute Pancreatic Injury in Mice and Rats

doi: 10.1097/MPA.0000000000001382

Figure Lengend Snippet: Rat Reg3γ Gene Expression Correlations to Reg3γ % Positivity and Histology Scores

Techniques: Expressing

Journal: Pancreas

Article Title: Detection of Reg3γ by Immunohistochemistry in Cerulein-Induced Model of Acute Pancreatic Injury in Mice and Rats

doi: 10.1097/MPA.0000000000001382

Figure Lengend Snippet: Rat Reg3γ Differential Gene Expression Statistics

Techniques: Expressing

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reg3γ antibody (WuXi AppTec)

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1) Product Images from "Detection of Reg3γ by Immunohistochemistry in Cerulein-Induced Model of Acute Pancreatic Injury in Mice and Rats"

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